This This study defined negativizing of positive treatment by using honorific prefix ｢オ⋅ご｣in Japanese language as the language behavior where speaker uses expression of plus treatment ｢オ⋅ご｣to signal his or her intention to give minus treatment to audience, and analyzed and examined the uses of it. In various ｢scenes｣, it was possible to identify uses of negativization of expression of positive treatment using ｢オ⋅ご｣. It was found that, in most cases, the prefix is used to express sarcasm in equal human relations as follows: ① While it is expression of plus treatment with honorific or polite words, we can grasp the intention of speaker to treat audience negatively by the context in which the prefix is used, human relations between speaker and audience, or the vocabulary used with ｢オ⋅ご｣; ② In the case where, even if ｢オ⋅ご｣is used, contents of the sentence is minus treatment, we can figure out the intention of speaker to express minus treatment without considering human relations or the context; ③ In the case where, while the sentence is expression of plus treatment, it is not formed as honorific expression without ｢オ⋅ご｣, we can recognize it as the expression of minus treatment by considering human relations or the context.It seems that the reason why speaker uses such an expression is to emphasize minus treatment of the audience, and to upgrade the effect of the expression. In most cases, audience seemed to understand the intention of speaker to express minus treatment.